The influence of oligonucleotide‐effector on the selectivity of sequence specific modification of 16 S rRNA

The influence of oligonucleotide‐effector on the selectivity of sequence specific modification of 16 S rRNA Научная публикация

Журнал

FEBS Letters
ISSN: 0014-5793 , E-ISSN: 1873-3468

Вых. Данные

Год: 1990, Том: 269, Номер: 1, Страницы: 26-28 Страниц : 3 DOI: 10.1016/0014-5793(90)81110-a

Авторы

Zenkova M.A. ¹ , Fedorova O.S. ¹ , Levina A.S. ¹ , Mamaev S.V. ¹ , Karpova G.G. ¹

Организации

1	Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of USSR Academy of Sciences, 630090 Novosibirsk, USSR

The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide-effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 μM effector at 5 μM concentration of the reagent.

Zenkova M.A. , Fedorova O.S. , Levina A.S. , Mamaev S.V. , Karpova G.G.
The influence of oligonucleotide‐effector on the selectivity of sequence specific modification of 16 S rRNA
FEBS Letters. 1990. V.269. N1. P.26-28. DOI: 10.1016/0014-5793(90)81110-a Scopus OpenAlex

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