Реферат: The previously suggested method of following ligand-receptor interactions by measuring ligand-induced changes in membrane fluidity (Manevich et al. 1986) was employed to study the binding of specific ligands of the muscarinic receptor to rat brain membrane fragments containing a fluorescent analog of phosphatidylcholine (APC) as a membrane probe. Upon addition of carbachol and atropine in low concentrations, the fluorescence polarization of the APC-labeled membranes decreased, demonstrating that binding of these ligands to the muscarinic receptor increases the fluidity of its lipid environment. The fluidity changes were specific, concentration-dependent and saturable. In comparison with radioligand assays, the fluorescent lipid probe method proved to be much more sensitive but the Kd values obtained by the 2 methods differed considerably.

Библиографическая ссылка: Manevich E.M. , Koiv A. , Jarv J. , Molotkovskii J.G. , Bergel'son L.D.
Binding of specific ligands to muscarinic receptors alters the fluidity of membrane fragments from rat brain. A fluorescence polarization study with lipid-specific probes
FEBS Letters. 1988. V.236. N1. P.43-46. DOI: 10.1016/0014-5793(88)80282-5 Scopus OpenAlex CAPlusCA PMID

Идентификаторы БД:

≡ Scopus:	2-s2.0-0023785335 Добавил Робот 22 янв. 2026 г.
≡ OpenAlex:	W2041031649 Добавил Робот 22 янв. 2026 г.
≡ Chemical Abstracts:	1988:523037 Добавил Зибарева И.В. 10 янв. 2026 г.
≡ Chemical Abstracts (print):	109:123037 Добавил Зибарева И.В. 10 янв. 2026 г.
≡ PMID (PubMed):	3402616 Добавил Зибарева И.В. 10 янв. 2026 г.

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