Biocatalytic Conversion of Semi-Finished Hardwood into Sugars: Enzymatic Hydrolysis at High Concentrations of the Substrate

Biocatalytic Conversion of Semi-Finished Hardwood into Sugars: Enzymatic Hydrolysis at High Concentrations of the Substrate Научная публикация

Журнал

Catalysis in Industry
ISSN: 2070-0504 , E-ISSN: 2070-0555

Вых. Данные

Год: 2024, Том: 16, Номер: 1, Страницы: 102–109 Страниц : DOI: 10.1134/s2070050424010070

Авторы

Semenova M.V. ¹ , Telitsin V.D. ² , Rozhkova A.M. ^1,2 , Kondratyeva E.G. ¹ , Shashkov I.A. ¹ , Satrutdinov A.D. ¹ , Gareev Ya.A. ³ , Moseev V.G. ⁴ , Kryazhev A.M. ³ , Sinitsyn A.P. ^1,2

Организации

1	Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences, Moscow, 119071 Russia
2	Moscow State University, Moscow, 119991 Russia
3	Tekhservis, Novodvinsk, 164900 Russia
4	Arkhangelsk Pulp & Paper Mill, Arkhangelsk, 164900 Russia

Exhaustive enzymatic hydrolysis is performed for semi-bleached sulfate hardwood cellulose (a semi-finished pulp and paper product) at ultra-high concentrations of it in a reaction mixture (up to 300 g/L per dry compound). Russian commercial enzyme preparations are used for hydrolysis. The best seems to be Agroxil Plus, which has high cellulase and endoxylanase activities. A total of 290 g/L of sugars (including 210 g/L of glucose and 30 g/L of xylose) is obtained using Agroxil Plus (20 mg protein/1 g substrate) in combination with an auxiliary β-glucosidase enzyme preparation (2 mg protein/1 g substrate) at an initial semi-bleached cellulose concentration of 300 g/L. The dosage of Agroxil Plus can be halved (10 mg of protein/1 g of substrate with a total concentration of semi-bleached cellulose of 300 g/L) with a high yield of hydrolysis product (270 g/L of sugars, including 200 g/L of glucose and 30 g/L of xylose), due to the fractional addition of a substrate.

Semenova M.V. , Telitsin V.D. , Rozhkova A.M. , Kondratyeva E.G. , Shashkov I.A. , Satrutdinov A.D. , Gareev Y.A. , Moseev V.G. , Kryazhev A.M. , Sinitsyn A.P.
Biocatalytic Conversion of Semi-Finished Hardwood into Sugars: Enzymatic Hydrolysis at High Concentrations of the Substrate
Catalysis in Industry. 2024. V.16. N1. P.102–109. DOI: 10.1134/s2070050424010070 WOS Scopus РИНЦ OpenAlex

Семенова М.В. , Телицин В.Д. , Рожкова А.М. , Кондратьева Е.Г. , Шашков И.А. , Сатрутдинов А.Д. , Гареева Я.А. , Мосеев В.Г. , Кряжев А.М. , Синицын А.П.
Биокаталитическое превращение полуфабриката лиственной древесины в сахара: проведение процесса ферментативного гидролиза при высокой концентрации субстрата
Катализ в промышленности. 2023. №4. С.75-83. DOI: 10.18412/1816-0387-2023-4-75-83 РИНЦ CAPlus OpenAlex

Поступила в редакцию:	5 дек. 2022 г.
Принята к публикации:	9 мар. 2023 г.
Опубликована в печати:	1 мар. 2024 г.

Web of science:	WOS:001221716600001
Scopus:	2-s2.0-85192951381
РИНЦ:	67307290
OpenAlex:	W4396852650