Effect of single‐point mutations Phe41→ His and Phe143→ Glu on folding and catalytic properties of recombinant horseradish peroxidase expressed in E. coli Научная публикация
| Журнал |
FEBS Letters
ISSN: 0014-5793 , E-ISSN: 1873-3468 |
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| Вых. Данные | Год: 1994, Том: 354, Номер: 3, Страницы: 248-250 Страниц : 3 DOI: 10.1016/0014-5793(94)01125-7 | ||
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Реферат:
Wild-type recombinant and Phe41→ His and Phe143→ Glu mutant forms of horseradish peroxidase have been expressed in E. coli and reactivated from inclusion bodies with a yield of about 25%. The purified homogeneous preparations have been studied in the reaction of ABTS oxidation. The effect of mutations on heme entrapment and kinetics of ABTS oxidation demonstrates the essential role of the replaced residues in providing the hydrophobic crevice for the non-covalent heme binding.
Библиографическая ссылка:
Gazaryan I.G.
, Doseeva V.V.
, Galkin A.G.
, Tishkov V.I.
Effect of single‐point mutations Phe41→ His and Phe143→ Glu on folding and catalytic properties of recombinant horseradish peroxidase expressed in E. coli
FEBS Letters. 1994. V.354. N3. P.248-250. DOI: 10.1016/0014-5793(94)01125-7 WOS Scopus OpenAlex
Effect of single‐point mutations Phe41→ His and Phe143→ Glu on folding and catalytic properties of recombinant horseradish peroxidase expressed in E. coli
FEBS Letters. 1994. V.354. N3. P.248-250. DOI: 10.1016/0014-5793(94)01125-7 WOS Scopus OpenAlex
Идентификаторы БД:
| ≡ Web of science: | WOS:A1994PT42900002 |
| ≡ Scopus: | 2-s2.0-0027942297 |
| ≡ OpenAlex: | W1970431680 |