Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field

Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field Научная публикация

Журнал

Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765

Вых. Данные

Год: 2014, Том: 125, Страницы: 104-109 Страниц : 6 DOI: 10.1016/j.colsurfb.2014.11.012

Авторы

Majouga Alexander ^1,2 , Sokolsky-Papkov Marina ³ , Kuznetsov Artem ¹ , Lebedev Dmitry ¹ , Efremova Maria ¹ , Beloglazkina Elena ¹ , Rudakovskaya Polina ¹ , Veselov Maxim ¹ , Zyk Nikolay ¹ , Golovin Yuri ^1,4 , Klyachko Natalia ^1,3 , Kabanov Alexander ^1,3

Организации

1	Laboratory of Chemical Design of Bionanomaterials, Chemistry Department, M.V. Lomonosov Moscow State University, Russian Federation
2	National University of Science and Technology MISiS, Leninsky Ave, 4, 119049 Moscow, Russian Federation
3	Center for Nanotechnology in Drug Delivery and Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
4	R.G. Derzhavin Tambov State University, Russian Federation

The possibility of remotely inducing a defined effect on NPs by means of electromagnetic radiation appears attractive. From a practical point of view, this effect opens horizons for remote control of drug release systems, as well as modulation of biochemical functions in cells. Gold-coated magnetite nanoparticles are perfect candidates for such application. Herein, we have successfully synthesized core–shell NPs having magnetite cores and gold shells modified with various sulphur containing ligands and developed a new, simple and robust procedure for the purification of the resulting nanoparticles. The carboxylic groups displayed at the surface of the NPs were utilized for NP conjugation with a model enzyme (ChT). In the present study, we report the effect of the low-frequency AC magnetic field on the catalytic activity of the immobilized ChT. We show that the enzyme activity decreases upon exposure of the NPs to the field.

Majouga A. , Sokolsky-Papkov M. , Kuznetsov A. , Lebedev D. , Efremova M. , Beloglazkina E. , Rudakovskaya P. , Veselov M. , Zyk N. , Golovin Y. , Klyachko N. , Kabanov A.
Enzyme-functionalized gold-coated magnetite nanoparticles as novel hybrid nanomaterials: Synthesis, purification and control of enzyme function by low-frequency magnetic field
Colloids and Surfaces B: Biointerfaces. 2014. V.125. P.104-109. DOI: 10.1016/j.colsurfb.2014.11.012 WOS

Web of science:

WOS:000348951400014