In vitro Effect of the Homocysteine Linker on Properties of Conjugates between Human Serum Albumin and Monomethyl Auristatin F

In vitro Effect of the Homocysteine Linker on Properties of Conjugates between Human Serum Albumin and Monomethyl Auristatin F Научная публикация

Журнал

Chemistry for Sustainable Development
ISSN: 1817-1818

Вых. Данные

Год: 2024, Том: 32, Номер: 6, Страницы: 750-759 Страниц : 10 DOI: 10.15372/CSD2024610

Авторы

Wang M. ^1,2 , Rogaleva V.I. ³ , Popova T.V. ^1,3 , Zakharova O.D. ³ , Godovikova T.S. ³ , Silnikov V.N. ^3,4

Организации

1	Novosibirsk State University, Novosibirsk, Russia
2	Fuzhou Fuyao Institute for Advanced Study, Fuzhou, China
3	Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia
4	NanoTech-S LLC, Novosibirsk, Russia

Fluorescent conjugates of human serum albumin and a maleimide derivative of monomethyl auristatin F containing the cleavable peptide linker ValCit were synthesised. Strategies included the direct attachment of the peptide to the albumin structure and the attachment of the peptide through the N-trifluoroacetylhomocysteine linker. Confocal microscopy showed that the conjugate with the homocysteine linker effectively accumulated in the cytoplasm of the MCF-7 tumor cells, whereas the conjugate without the linker displayed predominant surface accumulation. In vitro studies demonstrated that the monomethyl auristatin F conjugate exhibited enhanced cytotoxicity against MCF-7 and T98G cancer cells after its attachment to albumin. The homocysteine-containing conjugate showed superior cytotoxicity compared to the conjugate without the linker despite the lower peptide load.

Wang M. , Rogaleva V.I. , Popova T.V. , Zakharova O.D. , Godovikova T.S. , Silnikov V.N.
In vitro Effect of the Homocysteine Linker on Properties of Conjugates between Human Serum Albumin and Monomethyl Auristatin F
Chemistry for Sustainable Development. 2024. V.32. N6. P.750-759. DOI: 10.15372/CSD2024610 WOS РИНЦ OpenAlex

Ван М. , Рогалева В.И. , Попова Т.В. , Захарова О.Д. , Годовикова Т.С. , Сильников В.Н.
Изучение in vitro влияния гомоцистеинового линкера на поведение конъюгатов сывороточного альбумина человека и монометилауристатина F
Химия в интересах устойчивого развития. 2024. Т.32. №6. С.773-782. DOI: 10.15372/khur2024610 РИНЦ CAPlus OpenAlex

Поступила в редакцию:	2 нояб. 2024 г.
Принята к публикации:	20 нояб. 2024 г.
Опубликована online:	12 февр. 2025 г.

Web of science:	WOS:001419177900001
РИНЦ:	80313967
OpenAlex:	W4407634583